More than 3500 people are expected to attend a sold-out special expo at the velodrome after the bike ride, which will feature a one-off stunt show from trial cyclist Danny MacAskill and his former team, The Clan.
Scottish track cyclists Evan Oliphant and Charline Joiner will also perform a track display.
Outside the Emirates Arena there will be activities for families including a climbing wall, skate ramp and mountain bike skills track.
The family cycle will set off from the velodrome at 11am taking riders of all ages down London Road to Bridgeton Cross.
Lane closures will be in place between these two points until riders reach the segregated cycleway where London Road intersects with Kirkpatrick Street.
Riders exit Glasgow Green via Newhall Street and return to the velodrome on Clyde Gateway.
Newhall Street between James Street and Dunn Street and Main Street in Bridgeton between Rumford Street and Newhall Street will be closed from 8.30am until 1.30pm.
Ian Aitken, chief executive of Cycling Scotland, said: “I’m absolutely delighted to be kicking off our series of freshnlo Pedal for Scotland rides with a sold out velodrome.
“It will be the perfect warm up for the Glasgow to Edinburgh rides happening on Sunday, September 8, which are still open for entry.”
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